human circrna array v2 chip  (Arraystar inc)

Journal: Frontiers in Oncology

Article Title: CircRNA_036186 mediates HNSCC progression by regulating 14-3-3ζ

doi: 10.3389/fonc.2024.1498139

Figure Lengend Snippet: Identification of circRNA_036186-miR-193b-3p-14-3-3ζ-regulatory axis. (A) The heatmap illustrates the expression profiles of 287 circRNAs, of which 146 exhibited increased expression and 141 exhibited decreased expression. The colouration gradually transitions from blue to red, indicating increased expression. (B) The five most highly ranked circRNAs and their target miRNAs, each corresponding to a node, are shown with two interacting genes based on base sequence pairing connected by a solid line. (C) The intersection results of the HNSCC occurrence and development group, the prognosis group, and the competing endogenous RNA networks in OncomiR. (D) The predicted target mRNAs from three databases—Wayne ’ s map, miR-193b-3pmicroT-CDS, Tarbase, and TargetScan—were taken as intersections. (E) A search of the Tarbase database revealed that miR-193b-3p has a base sequence that binds and interacts with the 14-3-3ζ target. (F, G) The results of the RT-PCR experiments indicated that circRNA_036186 and miR-193b-3p exhibited elevated expression in HNSCC tissues relative to paracancerous tissues in five pairs of HNSCC and paracancerous tissue samples. (H, I) The results of RT-PCR experiments indicated that the mRNA expression of circRNA_036186 and miR-193b-3p was markedly elevated in all three HNSCC cell lines relative to HOK. (n=3, * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001).

Article Snippet: We used the Arratstar Super RNA Labeling Kit to amplify the enriched circRNA, which was then transcribed into fluorescent cRNA.The labelled cRNA was hybridised to the Arraystar Human circRNA V2 chip.

Techniques: Expressing, Sequencing, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Oncology

Article Title: CircRNA_036186 mediates HNSCC progression by regulating 14-3-3ζ

doi: 10.3389/fonc.2024.1498139

Figure Lengend Snippet: Top 5 circRNA.

Article Snippet: We used the Arratstar Super RNA Labeling Kit to amplify the enriched circRNA, which was then transcribed into fluorescent cRNA.The labelled cRNA was hybridised to the Arraystar Human circRNA V2 chip.

Techniques:

Journal: Frontiers in Oncology

Article Title: CircRNA_036186 mediates HNSCC progression by regulating 14-3-3ζ

doi: 10.3389/fonc.2024.1498139

Figure Lengend Snippet: Effect of down-regulation of circRNA_0361863p and miR-193b-3p on 14-3-3ζ in HSC2. (A) The results of the RT-PCR experiment indicated that si-circ_0036186 had been successfully transfected into HSC2. (B) The results of the RT-PCR experiments indicated that miR-193b-3p had been successfully transfected into HSC2. (C) Reverse transcription polymerase chain reaction (RT-PCR) experiments were conducted to ascertain the impact of transfection on 14-3-3ζ mRNA expression. (D, E) Western blotting experiments were conducted to ascertain the impact of transfection on 14-3-3ζ protein expression. (n=3, * p <0.05, *** p <0.001, **** p <0.0001).

Article Snippet: We used the Arratstar Super RNA Labeling Kit to amplify the enriched circRNA, which was then transcribed into fluorescent cRNA.The labelled cRNA was hybridised to the Arraystar Human circRNA V2 chip.

Techniques: Reverse Transcription Polymerase Chain Reaction, Transfection, Reverse Transcription, Polymerase Chain Reaction, Expressing, Western Blot

Journal: Frontiers in Oncology

Article Title: CircRNA_036186 mediates HNSCC progression by regulating 14-3-3ζ

doi: 10.3389/fonc.2024.1498139

Figure Lengend Snippet: Effect of down-regulation of circRNA_0361863p and miR-193b-3p on the proliferation, migration, invasion, and scratch healing rate of HSC2 cells. (A–D) The Transwell assay assessed the impact of circRNA_0361863p and miR-193b-3p down-regulation on cell migration and invasion of HSC2. (E, F) The Scratch test assessed the scratch healing rate of HSC2 after the down-regulation of circRNA_0361863p and miR-193b-3p. (G) The cell activity of HSC2 was evaluated after the down-regulation of circRNA_0361863p and miR-193b-3p through CCK-8 experiments. (n=3, **** p <0.0001).

Article Snippet: We used the Arratstar Super RNA Labeling Kit to amplify the enriched circRNA, which was then transcribed into fluorescent cRNA.The labelled cRNA was hybridised to the Arraystar Human circRNA V2 chip.

Techniques: Migration, Transwell Assay, Activity Assay, CCK-8 Assay

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Primer sequences used in the validation of circRNAs.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Biomarker Discovery, Sequencing

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: The top 15 upregulated and 15 downregulated circRNAs in gout patients compared with healthy control subjects.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Control

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Expression profiles of circRNAs in gout patients compared with healthy controls. (A) Scatter plot demonstrating the heterogeneity of the expression of circRNAs in the gout and HC groups. The values of the X and Y axes represent the averaged normalized signal values of the groups (log2-scaled). The green line represents 1.5-fold changes. The expression of circRNAs above the top green line and below the bottom green line indicates changes by > 2-fold between the two groups. (B) Volcano plots visualizing differentially expressed circRNAs between the two groups. The vertical lines correspond to 1.5-fold up- and downregulated circRNA expression. The horizontal line represents a p -value of 0.05. The red point in the plot represents significantly differentially expressed circRNAs. (C) Clustered heatmap showing the relationships among the expression levels of samples. Expression values are represented by the color scale. The intensity increases from green (relatively low expression) to red (relatively high expression). Each column represents one sample, and each row represents a single circRNA. (D) Genomic region distribution of upregulated circRNAs. (E) Genomic region distribution of downregulated circRNAs. (F) Distribution of significantly dysregulated circRNAs in chromosomes. (G) Relative expression levels of the six circRNAs in five gout patients and three healthy control (HC) subjects. The Y-axis represents the ratio of the relative expression level of circRNAs in the gout group to that of the HC group. Gout: primary gout; HC: healthy controls.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Expressing, Control

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Prediction and annotation of hsa_circRNA_103657 and hsa_circRNA_000241 ceRNA mechanism. (A) Construction of the circRNA-miRNA-mRNA network: the network of ceRNA includes 2 circRNAs, 10 miRNAs, and 525 mRNAs. (B) Schematic diagram of the gene category of the PI3K-Akt signaling pathway, which is the top enriched term in the KEGG pathway analysis. Image from the Kyoto Encyclopedia of Genes and Genomes (KEGG) resource ( http://www.genome.jp/kegg/ ).

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques:

Journal: Frontiers in Genetics

Article Title: Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

doi: 10.3389/fgene.2021.728091

Figure Lengend Snippet: Clinical significance of hsa_circRNA_103657 and hsa_circRNA_000241 in primary gout (A,B,C,D) Correlations between the expression levels of circRNAs (hsa_circRNA_103657 and hsa_circRNA_000241) and laboratory data of gout, analyzed using Spearman’s coefficient. (E, F) Receiver operating characteristic (ROC) curve for the analysis of the diagnostic value of hsa_circRNA_103657 and hsa_circRNA_000241 for primary gout. AUC: area under the curve.

Article Snippet: We used the Arraystar Human circRNA Array v2 chip (8 × 15 K) (Arraystar, Rockville, MD, United States), which contains 13,617 human circRNA probes.

Techniques: Expressing, Diagnostic Assay